PURPOSE: To obtain the titled antibiotic, by cultivating a novel species Streptomyces penemifaciens (FERM-P No. 5305), and collecting the titled antibiotic from the resultant culture.
CONSTITUTION: A novel species Streptomyces penemifaciens (FERM-P No. 5305) has the positive ability to hydrolyze starch and the negative ability to liquefy gelatine and will not grow at 10°C. The novel species is cultivated in an ordinary culture medium containing a carbon source, e.g. glucose, a nitrogen source, e.g. soybean flour, an inorganic, salt, etc. under aerobic conditions and ordinary cultivating conditions. The aimed thienamycin is obtained from the resultant culture by the ordinary methods, e.g. filtration, chromatography with an ion exchange resin, etc.